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Gamophyceae: Zygnematales: Desmidiaceae or Closteriaceae

 Closterium ralfsii
Brébisson var. ralfsii

Closterium ralfsii Species [var. ralfsii]: Cell body slightly curved, swelled at the center, gradually attenuated (cf. C. kuetzingii, C. setaceum) toward both slanted and narrow ends (cf. C. rostratum), 300-610 μm long, (30-)40-62 μm wide, L/W=6-9; cell wall brownish in color, lined (7-9/10 μm); 6-9 pyrenoids in each semicell.
[var. hybridum]: Cell body slender than var. ralfsii, slightly swelled at the center; 300-770 μm long, 24-40 μm wide, L/W=12-18.
[var. gracilius]: Cell body smaller, narrower than var. ralfsii, 140-242 μm long, 11-25 μm wide.
[var. novae-anguliae]: Cell body longe than var. ralfsii, (631-816)-1080 μm long, (23-26)-30 μm wide, L/W=28-31 (Illustrations of The Japanese Fresh-water Algae, 1977).
Similar species -->> C. kuetzingii; C. subulatum; C. rostratum;

Closterium ralfsii Brébisson var. ralfsii, cell body 390 μm long, 33 μm wide, L/W=11.8, x 200, x 400, x 640, a small pond near Yamakado marsh (Yamakado-Suigen-no-Mori), Nishi-Azai town, Shiga Pref., Japan, June 30, 2008, by Y. Tsukii
scale 50 μm scale 100 μm scale 150 μm; x 400 : scale 31 μm scale 63 μm scale 94 μm; x 640
Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii

Images of collecting locality: A small pond near Yamakado marsh (Yamakado-Suigen-no-Mori), Nishi-Azai town, Shiga Pref., Japan, June 30, 2008, by Y. Tsukii

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