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Gamophyceae: Zygnematales: Desmidiaceae or Closteriaceae

 Closterium ralfsii
Brébisson var. hybridum Rabenhorst

Closterium ralfsii Species [var. ralfsii]: Cell body slightly curved, swelled at the center, gradually attenuated (cf. C. kuetzingii, C. setaceum) toward both slanted and narrow ends (cf. C. rostratum), 300-610 μm long, (30-)40-62 μm wide, L/W=6-9; cell wall brownish in color, lined (7-9/10 μm); 6-9 pyrenoids in each semicell.
[var. hybridum]: Cell body slender than var. ralfsii, slightly swelled at the center; 300-770 μm long, 24-40 μm wide, L/W=12-18.
[var. gracilius]: Cell body smaller, narrower than var. ralfsii, 140-242 μm long, 11-25 μm wide.
[var. novae-anguliae]: Cell body longe than var. ralfsii, (631-816)-1080 μm long, (23-26)-30 μm wide, L/W=28-31 (Illustrations of The Japanese Fresh-water Algae, 1977).
Similar species -->> C. kuetzingii; C. subulatum; C. rostratum;

Closterium ralfsii Brébisson var. hybridum Rabenhorst or C. kuetzingii ?, cell body 434 μm long, 35 μm wide, L/W=12.4, x 200, x 400, x 640, a drainage ditch along northern side of Akaiyaji-shoya-shokubutu-gunraku (Akai wetland), Aizuwakamatsu city, Fukushima Pref., Japan, May 4, 2008 by Y. Tsukii
scale 50 μm scale 100 μm scale 150 μm; x 400 : scale 31 μm scale 63 μm scale 94 μm; x 640
Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii
Cell body μm long, μm wide, L/W=, x 200, x 400, x 640
Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii Closterium ralfsii

Images of collecting locality: A drainage ditch along northern side of Akaiyaji-shoya-shokubutu-gunraku (Akai wetland), Aizuwakamatsu city, Fukushima Pref., Japan, May 4, 2008 by Y. Tsukii

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