Genus: Trichome without mucous sheath; straight, long, consisting of numerous cells (Illustrations of The Japanese Fresh-water Algae, 1977).
Species: Trichome solitary or in small group, 1-1.5 μm wide; cell body 1-2 times longer than the width (L/W=1-2) (Illustrations of The Japanese Fresh-water Algae, 1977).

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O. subtilissima 1.5 μm φ L/W=1.0-1.7 Ageo Maruyama Park Saitama, 2002	O. subtilissima ? 1.5-3.5 x 1.5 μm φ L/W=1.0-2.3 Chiba-Minato Park Chiba, 2003	O. subtilissima ? 2-4 x 1.5 μm L/W=1.3-2.7 Mizuko Fujimi Saitama, 2004	O. subtilissima ? 2.5-4 x 1.5 μm L/W=1.7-2.7 Ichinose-enchi Norikura highland Nagano, 2005
O. subtilissima ? 2-4 x 2 μm L/W= Furu-tone river Kasukabe Saitama, 2004	O. subtilissima 1.5-3 x 2.5 μm L/W= Furu-tone river Kasukabe Saitama, 2004	O. subtilissima 2-4.5 x 2 μm L/W= Watarase river Ashikaga Tochigi, 2004	O. subtilissima 2.7 x 2 μm L/W= Showa m.p. Tachikawa-ku Tokyo, 2001
O. subtilissima 2-3 x 2-3 μm L/W= Tsukamoto Sakura-ku Saitama, 2004	O. subtilissima 3-6 x 2 μm L/W= Ikenokochi marsh Tsuruga Fukui, 2004	O. subtilissima 3-6 x 2 μm L/W= Omotsubo marsh Tetta-cho Okayama, 2004	O. subtilissima 2-5 x 2 μm L/W=1.3-2.5 Shiba river near Owada Park Saitama, 2005
O. subtilissima 2-3 x 2 μm L/W=1.0-1.5 Tanohara Natural p. Ontake highland Nagano, 2005

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