Oligohymenophorea
(formerly Nassophorea): Peniculida: Parameciidae
Paramecium caudatum
Ehrenberg, 1838
Cells stained with Azure C
Method: Cells dried on a slide glass; fixed with ethanol : acetic acid (3 : 1) for 10 min;
pretreated with HCl (6N, 10 min); washed with distilled (or deionized) water (5min x 2); stained
with azure C (about 0.1 mg/ml, 10~15 min); washed with distilled water for 2-3 sec; air dried
P. caudatum strain unknown; a vegetative cell; azure C staining pretreated with HCl,
by Y. Tsukii
Cytoplasmic RNAs removed, only food vacuole stained in cytoplasm
P. caudatum strain unknown; a vegetative cell; azure C staining without HCl pretreatment,
by Y. Tsukii
Cytoplasmic RNAs remained and stained with azure C
P. caudatum strain Nn 4a; a vegetative cell; azure C staining without HCl pretreatment,
by Y. Tsukii
Macronulcear inclusion bodies conspicous
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