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Oligohymenophorea (formerly Nassophorea): Peniculida: Parameciidae

Paramecium caudatum
Ehrenberg, 1838

Macronuclear inclusion body

Paramecium caudatum macronuclear inclusion body Genus: Cigar- or foot-shaped; a single macronucleus and 1 to several vesicular or compact micronuclei (Kudo, 1966).
Species: 180-300 μm long; a compact micronucleus; two contractile vacuoles (Kudo, 1966). Cylindrical or cigar-shaped; rounded anteriorly, pointed posteriorly; one large compact micronucleus ("caudatum" group) approximately 8 μm diam.; two contractile vacuoles; 170-290 μm long (Wichterman, 1986). Elongate, rounded anteriorly, pointed posteriorly; caudal cilia present; 180-280 μm long; a single macronucleus with a single micronucleus; two contractile vacuoles (Carey, 1992).

A list of endosymbionts, strain Nn 4a, by Y. Tsukii
A scheme for the transplantation experiment for the macronuclear inclusion bodies; by Y. Tsukii
Differences between the inclusion bodies and nucleoli; by Y. Tsukii
A scheme for the experiments proving endosymbiotic nature of the inclusion bodies; by Y. Tsukii
A protocol for isolating macronuclear inclusion bodies; by Y. Tsukii
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An intact cell containing inclusion body (P. caudatum strain Nn 4a; x2); by Y. Tsukii
Inclusion bodies stained with aceto-fastgreen (Nn 4a, P. caudatum); by Y. Tsukii
Cells containing inclusion bodies; by Y. Tsukii
Inclusion bodies at mac. fragmentation, phase-contrast image; by Y. Tsukii
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Vegetative cell; azure C staining pretreated with HCl; by Y. Tsukii
A cell containing inclusion bodies, stained with azure C pretreated with HCl; by Y. Tsukii
Vegetative cell; azure C staining without HCl pretreatment; by Y. Tsukii
Mac. containing inclusion body (azure C stain; Nn 4a, P. caudatum); by Y. Tsukii
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A cell containing inclusion body double stained with aceto-orcein and fastgreen; by Y. Tsukii
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Steps of isolation of inclusion bodies; by Y. Tsukii
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An isolated macronucleus (Nn 4a, P. caudatum); by Y. Tsukii
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An isolated macronucleus stained with aceto-fastgreen; by Y. Tsukii
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An isolated macronucleus (swelled mac.); by Y. Tsukii
Paramecium caudatum, macronuclear inclusion body

An isolated inclusion bodies x4; stained with aceto-fastgreen; by Y. Tsukii
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PAGE of the isolated inclusion bodies and mac. ; by Y. Tsukii
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Cells containing inclusion bodies, after dispersion of the inclusions; by Y. Tsukii
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PAGE of the isolated mac. (before and after their dispersion); by Y. Tsukii
Paramecium caudatum, macronuclear inclusion body


EM of the mac. containing inclusion bodies (x1); inclusion bodies (x2); by K. Yazaki
EM of the isolated mac. containing inclusion bodies (x2); by K. Yazaki
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